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Your Position: Home > Protein > HLA-A*0301 & B2M > HLM-H82W6

Biotinylated Human HLA-A*03:01&B2M Monomer Protein (Peptide free, MALS verified)

Streptavidin Protein - PE (STN-NP117, STN-NP119), supporting the assembly of your own MHC Tetramers!

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  • Source
    Biotinylated Human HLA-A*03:01&B2M Monomer Protein(HLM-H82W6) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M) (Accession # P04439-1 (HLA-A*03:01) & P61769 (B2M)).
    Predicted N-terminus: Gly 25 & Ile 21
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 35.9 kDa and 11.7 kDa. The protein migrates as 40-43 kDa and 14 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HLA-A*0301 & B2M SDS-PAGE

Biotinylated Human HLA-A*03:01&B2M Monomer Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
HLA-A*0301 & B2M MALS images

The purity of Biotinylated Human HLA-A*03:01&B2M Monomer Protein (Cat. No. HLM-H82W6) is more than 90% and the molecular weight of this protein is around 44-64 kDa verified by SEC-MALS.

Bioactivity-ELISA
 HLA-A*0301 & B2M ELISA

Immobilized Biotinylated Human HLA-A*03:01&B2M Monomer Protein (Cat. No. HLM-H82W6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-1 ng/mL (QC tested).

Bioactivity-Stability
 HLA-A*0301 & B2M STABILITY

The complex protein was verified stable after incubation under 25 degrees for 48 hours, and no significant dissociation of HLA-A*0301 and B2M was observed from HPLC-SEC analysis.

  • Background
    Major histocompatibility complex (MHC), group of genes that code for proteins found on the surfaces of cells that help the immune system recognize foreign substances. MHC proteins are found in all higher vertebrates. In human beings the complex is also called the human leukocyte antigen (HLA) system.
  • Clinical and Translational Updates

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