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Your Position: Home > Kits > Spike RBD > RAS-A146

SARS-CoV-2 Spike RBD (XBB.1.5) ELISA Kit (For Vaccine Development)

For research use only.

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RAS146-C01Pre-coated Anti-SARS-CoV-2 Spike RBD Antibody Microplate1 plate
RAS146-C02SARS-CoV-2 Spike RBD (XBB.1.5)15 μg
RAS146-C03Biotin-Anti-SARS-CoV-2 Spike RBD Antibody100 μL
RAS146-C04Streptavidin-HRP10 μg
RAS146-C0510xWashing Buffer50 mL
RAS146-C06Dilution Buffer50 mL
RAS146-C07Substrate Solution12 mL
RAS146-C08Stop Solution7 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of SARS-CoV-2 Spike RBD is urgently needed to accelerate the development of COVID-19 vaccines.
  • Application

    This kit is developed for quantitative detection of SARS-CoV-2 Spike RBD (XBB.1.5) in samples.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Spike RBD. The kit consists of microplate pre-coated with Anti-SARS-CoV-2 Spike RBD Antibody, an SARS-CoV-2 Spike RBD as Control,biotin-Anti-SARS-CoV-2 Spike RBD Antibody, HRP-Streptavidin and buffers.

    Your experiment will include 6 simple steps:

    a) Bring all reagents and samples to room temperature (20℃-25℃) before use.

    b) Add your sample to the plate, take the SARS-CoV-2 Spike RBD as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    c) Add the Secondary antibody biotin-Anti-SARS-CoV-2 Spike RBD Antibody diluted by Dilution Buffer to the plate.

    d) Add the diluted Streptavidin-HRP to the plate.

    e) Wash the plate and add TMB or other colorimetric HRP substrate.

    f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

Typical Data Please refer to Ds document for the assay protocol.

Detection of SARS-CoV-2 Spike RBD (XBB.1.5) by sandwich-ELISA Assay.
Immobilized Anti-SARS-CoV-2 Spike RBD Antibody can bind SARS-CoV-2 Spike RBD (XBB.1.5). Detection was performed using Biotin-Anti-SARS-CoV-2 Spike RBD Antibody with sensitivity of 1.56 ng/mL (QC tested).

  • Clinical and Translational Updates

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Price(USD) : $780.00

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