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Your Position: Home > Viral Proteins > Hemagglutinin (HA) > HA9-V5227

Influenza A [A/Shanghai/2/2013(H7N9)] Hemagglutinin (HA) Protein, His Tag

  • Synonym
    HA,Hemagglutinin
  • Source
    Influenza A [A/Shanghai/2/2013(H7N9)] HA, His Tag (HA9-V5227) is expressed from human 293 cells (HEK293). It contains AA Asp 19 - Gly 338, Leu 341 - Asp 523 (Accession # R4NN21-1).
    Predicted N-terminus: Asp 19
  • Molecular Characterization
    Online(Asp 19 - Gly 338, Leu 341 - Asp 523) R4NN21-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 56.7 kDa. The protein migrates as 63-75 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Influenza A [A/Shanghai/2/2013(H7N9)] HA, His Tag (Cat. No. HA9-V5227) SDS-PAGE gel

Influenza A [A/Shanghai/2/2013(H7N9)] HA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Influenza A [A/Shanghai/2/2013(H7N9)] HA, His TagInfluenza A [A/Shanghai/2/2013(H7N9)] HA, His Tag (Cat. No. HA9-V5227) ELISA bioactivity

Immobilized Influenza A [A/Shanghai/2/2013(H7N9)] HA, His Tag (Cat. No. HA9-V5227) at 2 μg/mL (100 μL/well) can bind Influenza A H7N9 Hemagglutinin (HA) Affinity Purified Polyclonal Ab, Rabbit IgG with a linear range of 0.006-0.1 μg/mL (QC tested).

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