Select Language
Free Registration

IL-2 and IL-2 Receptor Proteins

Interleukin-2 (IL-2) is a pluripotent cytokine which plays a crucial role in the immune system response. And the IL-2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, that binds and responds to IL-2.
IL-2R consists of three subunits, namely IL-2Rα (CD25), IL-2Rβ (CD122), and common γc (CD132). The three receptor chains are expressed separately and differently on various cell types and can assemble in different combinations and orders to generate low, intermediate, and high affinity IL-2 receptors.
Many biopharma and biotech companies have carried out different IL-2 modification and design, including PEG modification, fusion Fc, IL-2 mutant design, bispecific antibody design, and combined immunization checkpoint antibody drug therapy and other strategies.
Therefore, a series of structurally stable and high-affinity IL-2 receptor heterodimers and heterotrimers are of great significance for in vitro studies of the interaction between IL-2 and IL-2 receptors, and also for the antibody immunization and screening.
Native IL-2 has pleiotropic effects on the immune response

Fig.1 Native IL-2 has pleiotropic effects on the immune response[1]

Schematic of IL-2 binding to the low-affinity, intermediate-affinity and high-affinity IL-2 receptors

Fig 2. Schematic of IL-2 binding to the low-affinity, intermediate-affinity and high-affinity IL-2 receptors. The high-affinity receptor comprises IL-2 receptor α-chain (IL-2Rα), IL-2Rβ and IL-2Rγ.[2]

ACROBiosystems has developed a series of IL-2 related proteins: IL-2, IL-2Rα, IL-2Rβ, IL-2Rγ, IL-2R βγ heterodimer, and IL-2Rαβγ heterotrimer with high purity, high stability and high bioactivity, which can be used to research the interaction between IL-2 and IL-2 receptors, immunization, antibody screening, etc., to facilitate the development of IL-2 related drugs. In addition, we can also provide GMP-grade IL-2 used for scale-up culturing of various cell types such as T/NK cells and iPSCs.

Features

Product list

IL-2
IL-2Rα
IL-2Rβ
IL-2Rγ
IL-2Rβγ
IL-2Rαβγ

More IL series products

Assay data

Affinity Verified by SPR
The affinity verification results of IL-2 with IL-2 receptor by SPR are basically consistent with the literature[4]

Table 1 Affinity Data of IL-2 Binding to IL-2 Receptor

IL-2 R

IL-2 R αβγ

IL-2 R βγ

IL-2 R alpha

IL-2 R beta

Affinity to Human IL-2

40.6 pM

0.279 nM

29.9 nM

377 nM

Affinity verification data of IL-2 binding to IL-2 receptor protein (SPR)

Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag (Cat. No. ILG-H5257) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-2, Tag Free with an affinity constant of 40.6 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

Affinity verification data of IL-2 binding to IL-2 receptor protein (SPR)

Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILG-H5254) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-2, Tag Free with an affinity constant of 0.279 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

Affinity verification data of IL-2 binding to IL-2 receptor protein (SPR)

Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) captured on CM5 chip via anti-His antibody, can bind Human IL-2, Tag Free with an affinity constant of 29.9 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

Affinity verification data of IL-2 binding to IL-2 receptor protein (SPR)

Cynomolgus IL-2 R beta, His Tag (Cat. No. ILB-C52H9) immobilized on CM5 Chip can bind Cynomolgus IL-2, His Tag (Cat. No. IL2-C5249) with an affinity constant of 377 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

Heterogeneity Verified by MALS
MALS verification of IL-2RB&IL-2RG heterodimer protein

The purity of Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)(Cat. No. ILG-H5254) is more than 90% and the molecular weight of this protein is around 145-165 kDa verified by SEC-MALS.

MALS verification of IL-2RB&IL-2RA&IL-2RG heterotrimer protein

The purity of Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag(Cat. No. ILG-H5257) is more than 90% and the molecular weight of this protein is around 175-190 kDa verified by SEC-MALS.

Bioactivity Verified by ELISA
Verification data of IL-2 binding to IL-2 receptor protein (ELISA)

Immobilized Biotinylated Human IL-2, His,Avitag (Cat. No. IL2-H82E4) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag (Cat. No. ILG-H5257) with a linear range of 0.4-6 ng/mL (QC tested).

Protocol

Verification data of IL-2 binding to IL-2 receptor protein (ELISA)

Immobilized Biotinylated Human IL-2, His,Avitag (Cat. No. IL2-H82E4) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag (Cat. No. ILG-H5257) with a linear range of 0.4-6 ng/mL.

Protocol

References

1. NKTR-2017JPMorganSlides

2. Spolski R, et al. Nat Rev Immunol. 2018. PMID: 30089912 Review.

3. Xinquan Wang et al.,2005, Science. 310(5751):1159-63.

4. Balasubramanian, S. Int Immunol. 1995 Nov;7(11):1839-49.

  • Background
  • Features
  • Product list
  • More IL series products
  • Assay data
  • References