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The newly identified 2019 novel coronavirus (SARS-CoV-2) has posed a serious threat to human health, and it spreads quickly.This kit is developed for detection of IgG titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
This kit is developed for detection of titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
The unopened kit is stable for at least 1 year from the date of manufacture if stored at 2°C to 8°C. The opened kit is stable for up to1 months from the date of opening.
It is recommended not to freeze thaw more than 3 times.
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of anti-SARS-CoV-2 IgG in serum by SARS-CoV-2 Spike Protein. The kit consists of High-bind Plate, SARS-CoV-2 Spike Protein, an Anti-SARS-CoV-2 Antibody (Control, IgG), an HRP-Anti-Human IgG secondary antibody and related buffer.
Your experiment will include 6 simple steps: a) Coat the plate with SARS-CoV-2 Spike Protein.
b) Wash the plate with universal ELISA buffer and block the plate with the blocking buffer.
c) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Blocking / Dilution Buffer.
d) Add a diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Blocking / Dilution Buffer.
e) Wash the plate and add TMB or other colorimetric HRP substrate.
f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
Detection of Anti-SARS-CoV-2 Antibody, Human IgG1 titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike Protein at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 in 1:50 human serum. Detection was performed using HRP-Conjugated Anti-human IgG antibody with sensitivity of 12 ng/mL (QC tested).
Protocol