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The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective Assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.
This kit is developed for serologic test for IgG titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
It is for research use only.
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Upon receipt, please store the lyophilized products at -20°C. After reconstitution, the stock solution should be kept at -70°C.
It is recommended not to freeze thaw more than 3 times.
This product is stable under storage conditions: 12 months in sealed state; Used within 3 months after opening.
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in serum by SARS-CoV-2 Spike protein RBD. The Kit consists of High-bind Plate, Spike protein RBD, an Anti-SARS-CoV-2 Antibody (Control, IgG), an HRP-Anti-Human IgG secondary antibody and Blocking/Dilution buffer.
Your experiment will include 6 simple steps:
a) Coat the plate with SARS-CoV-2 Spike protein RBD.
b) Wash the plate with universal ELISA buffer and block the plate with the blocking buffer.
c) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Blocking / Dilution Buffer.
d) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Blocking / Dilution Buffer.
e) Wash the plate and add TMB or other colorimetric HRP substrate.
f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
Detection of Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 S protein RBD at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 in 1:50 human serum. Detection was performed using HRP-Conjugated Anti-human IgG antibody with sensitivity of 24 ng/mL (QC tested).
Protocol