Immobilized Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Human ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52Wa) with a linear range of 0.02-0.313 μg/mL (QC tested).
Human integrin alpha(10)I domain as a recombinant protein to reveal its ligand binding specificity. In general, alpha(10)I did recognize collagen types I-VI and laminin-1 in a Mg(2+)-dependent manner, whereas its binding to tenascin was only slightly better than to albumin. Alpha 10 beta 1 is a known collagen-binding I domain integrin, in addition to ?1?1, ?2?1 and ?11?1. GROGER found in the N-terminal domain of collagens I and III, is only weakly recognised by ?10?1, an important collagen receptor on chondrocytes, contrasting with the other collagen-binding integrins.