This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 53.8 kDa. The protein migrates as 85-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
HIV-1 [HIV-1/Clade B/C (CN54)] GP120, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized HIV-1 [HIV-1/Clade B/C (CN54)] GP120, His Tag (Cat. No. GP4-V15227) at 5 μg/mL (100 μL/well) can bind Human CD4, Fc Tag (Cat. No. CD4-H5259) with a linear range of 1-39 ng/mL (QC tested).
Authors: S Sarkar, et al
Journal: Vaccine 43545
Enabling electrical biomolecular detection in high ionic concentrations and enhancement of the detection limit thereof by coupling a nanofluidic crystal with reconfigurable ion concentration polarization
Authors: Ouyang W, et al.
Journal: Lab Chip 2017
Authors: Beduleva L, et al.
Journal: AIDS Res Hum Retroviruses 2016
Application: Immunization & ELISA
Authors: Rao VR, et al.
Journal: PLoS One 2014
Application: Cell Culture
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