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Your Position: Home > All Other Proteins > DLL4 > DL4-H5259

Human DLL4 Protein, Fc Tag

  • Synonym
    DLL4,Delta4
  • Source
    Human DLL4, Fc Tag (DL4-H5259) is expressed from human 293 cells (HEK293). It contains AA Ser 27 - Pro 524 (Accession # NP_061947.1).
    Predicted N-terminus: Ser 27
  • Molecular Characterization
    Online(Ser 27 - Pro 524) NP_061947.1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 80.9 kDa. The protein migrates as 95-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human DLL4, Fc Tag (Cat. No. DL4-H5259) SDS-PAGE gel

Human DLL4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human DLL4, Fc TagHuman DLL4, Fc Tag (Cat. No. DL4-H5259) ELISA bioactivity

Immobilized Human DLL4, Fc Tag (Cat. No. DL4-H5259) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human DLL4 Antibody, Human IgG1 with a linear range of 1-16 ng/mL (QC tested).

Human DLL4, Fc TagHuman DLL4, Fc Tag (Cat. No. DL4-H5259) ELISA bioactivity

Immobilized Mouse NOTCH1, His Tag (Cat. No. NO1-M52H8) at 2 μg/mL (100 μL/well) can bind Human DLL4, Fc Tag (Cat. No. DL4-H5259) with a linear range of 0.5-8 ng/mL (Routinely tested).

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Datasheet & Documentation

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DMF (Drug Master File)

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