This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 61.7 kDa. The protein migrates as 66-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Glypican 3 (S359F), His Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 0.5 μg/mL (100 μL/well) can bind Human Glypican 3 (S359F), His Tag, low endotoxin (Cat. No. GP3-H5223) with a linear range of 3-128 ng/mL (QC tested).
Anti-Human GPC3 MAb (chimeric mouse-human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human Glypican 3 (S359F), His Tag, low endotoxin (Cat. No. GP3-H5223) with an affinity constant of 1.96 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Anti-Human GPC3 MAb (chimeric mouse-human IgG1) on AHC Biosensor, can bind Human Glypican 3 (S359F), His Tag, low endotoxin (Cat. No. GP3-H5223) with an affinity constant of 3.04 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
293 cells were transfected with anti-GPC3-scFv and RFP tag. 2e5 of the cells were first stained with B. Human Glypican 3 (S359F), His Tag, low endotoxin (Cat. No. GP3-H5223, 3 μg/mL) and C. His tag protein control, followed by FITC-conjugated anti-6xHis tag antibody. A. Non-transfected 293 cells and C. His tag protein control were used as negative control. RFP was used to evaluate CAR (anti-GPC3-scFv) expression and FITC was used to evaluate the binding activity of Human Glypican 3 (S359F), His Tag, low endotoxin (Cat. No. GP3-H5223).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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