0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > All Other Proteins > DLL4 > DL4-H5227

Human DLL4 Protein, His Tag

  • Synonym
    DLL4,Delta4
  • Source
    Human DLL4, His Tag (DL4-H5227) is expressed from human 293 cells (HEK293). It contains AA Ser 27 - Pro 524 (Accession # Q9NR61-1).
    Predicted N-terminus: Ser 27
  • Molecular Characterization
    Online(Ser 27 - Pro 524) Q9NR61-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 55.5 kDa. The protein migrates as 58-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human DLL4, His Tag (Cat. No. DL4-H5227) SDS-PAGE gel

Human DLL4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human DLL4, His TagHuman DLL4, His Tag (Cat. No. DL4-H5227) ELISA bioactivity

Immobilized Human DLL4, His Tag (Cat. No. DL4-H5227) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human DLL4 Antibody, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested).

Human DLL4, His TagHuman DLL4, His Tag (Cat. No. DL4-H5227) ELISA bioactivity

Immobilized Mouse Notch-1 Protein ECD, Fc Tag at 2 μg/mL (100 μL/well) can bind Human DLL4, His Tag (Cat. No. DL4-H5227) with a linear range of 0.039-0.313 μg/mL (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human NOTCH2, Fc Tag (Cat. No. NO2-H5255) on Protein A Biosensor, can bind Human DLL4, His Tag (Cat. No. DL4-H5227) with an affinity constant of 77.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Human NOTCH1, Fc Tag (Cat. No. NO1-H5255) on Protein A Biosensor, can bind Human DLL4, His Tag (Cat. No. DL4-H5227) with an affinity constant of 67.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Delta-like protein 4 (DLL4) is also known as Drosophila Delta homolog 4 (Delta4), which contains one DSL domain and eight EGF-like domains. DLL4 is expressed in vascular endothelium. DLL4 is involved in the Notch signaling pathway as Notch ligand, which can activates NOTCH1 and NOTCH4. DLL4 is involved in angiogenesis and negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. DLL4 can bind to Notch-1 and Notch-4.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)

Order Details

Price(USD) : $250.00

Price(USD) : $1400.00

Promotion & Exhibitions



Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:3 Details
  • Latest Research Phase:Phase 2 Clinical

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Questions & Comments

This web search service is supported by Google Inc.

totop