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Your Position: Home > Immune Checkpoint Proteins > BTLA > BTA-H5255

Human BTLA (31-134) Protein, Fc Tag

  • Synonym
    BTLA,CD272
  • Source
    Human BTLA, Fc Tag (BTA-H5255) is expressed from human 293 cells (HEK293). It contains AA Lys 31 - Thr 134 (Accession # NP_001078826.1).
    Predicted N-terminus: Lys 31
  • Molecular Characterization
    Online(Lys 31 - Thr 134) NP_001078826.1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 38.7 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >92% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human BTLA, Fc Tag (Cat. No. BTA-H5255) SDS-PAGE gel

Human BTLA, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity-ELISA
Human BTLA, Fc TagHuman BTLA, Fc Tag (Cat. No. BTA-H5255) ELISA bioactivity

Immobilized Human HVEM, His Tag (Cat.No. HVM-H52E9) at 5 μg/mL (100 μL/well) can bind Human BTLA, Fc Tag (Cat.No. BTA-H5255) with a linear range of 0.08-0.3 μg/mL (QC tested).

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