This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 18.5 kDa. The protein migrates as 32-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IL-18BP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human IL-18BP, His Tag(Cat. No. ILP-H5222) is more than 90% and the molecular weight of this protein is around 32-45 kDa verified by SEC-MALS.
Human IL-18BP, His Tag (Cat. No. ILP-H5222) inhibits the IL-18-induced IFN-gamma secretion by KG‑1 cells. The ED50 for this effect is 0.019-0.053 µg/mL in the presence of 30 ng/mL of Recombinant Human IL‑18 (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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