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Your Position: Home > All Other Proteins > IL-18BP > ILP-H5222

Human IL-18BP Protein, His Tag (MALS verified)

  • Synonym
    IL18BP,IL18BPa,Tadekinig-alfa
  • Source
    Human IL-18BP, His Tag (ILP-H5222) is expressed from human 293 cells (HEK293). It contains AA Thr 31 - Gly 194 (Accession # NP_001138529).
    Predicted N-terminus: Thr 31
  • Molecular Characterization
    Online(Thr 31 - Gly 194) NP_001138529

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 18.5 kDa. The protein migrates as 32-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IL-18BP, His Tag (Cat. No. ILP-H5222) SDS-PAGE gel

Human IL-18BP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human IL-18BP, His Tag (Cat. No. ) MALS images

The purity of Human IL-18BP, His Tag(Cat. No. ILP-H5222) was more than 90% and the molecular weight of this protein is around 32-45 kDa verified by SEC-MALS.

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Human IL-18BP, His Tag (Cat. No. ILP-H5222) inhibits the IL-18-induced IFN-gamma secretion by KG‑1 cells. The ED50 for this effect is 0.019-0.053 µg/mL in the presence of 30 ng/mL of Recombinant Human IL‑18 (Routinely tested).

  • Background
    Interleukin-18-binding protein (IL18BP) is also known as Tadekinig-alfa, IL18BPa, which functions as an IL18 inhibitor. IL18BP is a secreted protein which contains one Ig-like C2-type (immunoglobulin-like) domain. IL18BP does not have a transmembrane domain and hence is not anchored to the cell membrane. IL18BP is strongly expressed in heart, lung, placenta and spleen. Isoform A of IL18BP binds to IL-18 and inhibits its activity. IL18BP also functions as an inhibitor of the early TH1 cytokine response. IL18BP suppresses the production of IFN-gamma resulting in reduced T-helper type 1 immune responses.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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