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Your Position: Home > All Other Proteins > CD3E & CD3D > CDD-C52W4

Cynomolgus CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (MALS verified)

  • Synonym
    CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta / epsilon
  • Source
    Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (CDD-C52W4) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # Q95LI5(CD3E) & Q95LI8(CD3D)).
    Predicted N-terminus: Gln 22 (CD3E) & Phe 22 (CD3D)
  • Molecular Characterization
    Online(Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D)) Q95LI5 (CD3E) & Q95LI8 (CD3D)

    Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (MALS verified) is produced by co-expression of CD3E and CD3D, has a calculated MW of 15.5 kDa (CD3E) and 14.5 kDa (CD3D). Subunit CD3E is fused with his tag at the C-terminus and subunit CD3D is fused with flag tag at the C-terminus. The predicted N-terminus is Gln 22 (CD3E) and Phe 22 (CD3D). The reducing (R) heterodimer protein migrates as 19-22 kDa and 25-40 kDa due to glyosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (Cat. No. CDD-C52W4) SDS-PAGE gel

Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (Cat. No. ) MALS images

The purity of Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (Cat. No.CDD-C52W4) was more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.

Bioactivity-ELISA
Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag TagCynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (Cat. No. CDD-C52W4) ELISA bioactivity

Immobilized Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag & Flag Tag (Cat. No. CDD-C52W4) at 2 μg/mL, add increasing concentrations of Bispecific T cell Engager (CD3 X BCMA) and then add Biotinylated BCMA Fc,Avitag (Cat. No. BC7-H82F0) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.2 ng/mL (Intact assay, QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Bispecific T-cell Engager (CD3 X BCMA) immobilized on CM5 Chip can bind Cynomolgus CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (Cat. No. CDD-C52W4) with an affinity constant of 64.4 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human CD3×BCMA scFV on AHC Biosensor via DMF Filed Human BCMA, Fc Tag (Cat. No. BC7-H5254), can bind Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (Cat. No. CDD-C52W4) with an affinity constant of 8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Price(USD) : $350.00

Price(USD) : $1980.00

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