Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (MALS verified) is produced by co-expression of CD3E and CD3D, has a calculated MW of 15.5 kDa (CD3E) and 14.5 kDa (CD3D). Subunit CD3E is fused with his tag at the C-terminus and subunit CD3D is fused with flag tag at the C-terminus. The predicted N-terminus is Gln 22 (CD3E) and Phe 22 (CD3D). The reducing (R) heterodimer protein migrates as 19-22 kDa and 25-40 kDa due to glyosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (Cat. No.CDD-C52W4) was more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.
Immobilized Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag & Flag Tag (Cat. No. CDD-C52W4) at 2 μg/mL, add increasing concentrations of Bispecific T cell Engager (CD3 X BCMA) and then add Biotinylated BCMA Fc,Avitag (Cat. No. BC7-H82F0) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.2 ng/mL (Intact assay, QC tested).
Bispecific T-cell Engager (CD3 X BCMA) immobilized on CM5 Chip can bind Cynomolgus CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (Cat. No. CDD-C52W4) with an affinity constant of 64.4 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Human CD3×BCMA scFV on AHC Biosensor via DMF Filed Human BCMA, Fc Tag (Cat. No. BC7-H5254), can bind Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (Cat. No. CDD-C52W4) with an affinity constant of 8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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