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Your Position: Home > All Other Proteins > LIF R > Human-LIFR-CD118-Protein-C-Fc Tag

Human LIF R / CD118 Protein, Fc Tag

  • Synonym
    LIFR,CD118,FLJ98106,FLJ99923,SJS2,STWS,SWS
  • Source
    Human LIF R, Fc Tag (LIR-H4252) is expressed from human 293 cells (HEK293). It contains AA Gln 45 - Ser 833 (Accession # NP_001121143).
    Predicted N-terminus: Gln 45
  • Molecular Characterization
    Online(Gln 45 - Ser 833) NP_001121143

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 115.5 kDa. The protein migrates as 140-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human LIF R, Fc Tag (Cat. No. LIR-H4252) SDS-PAGE gel

Human LIF R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human LIF R, Fc TagHuman LIF R, Fc Tag (Cat. No. LIR-H4252) ELISA bioactivity

Immobilized Human LIF, Tag Free (Cat. No. LIF-H521b) at 0.2μg/mL (100 μL/well) can bind Human LIF R, Fc Tag (Cat. No. LIR-H4252) with a linear range of 7.8-31.25 ng/mL (QC tested).

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