0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > All Other Proteins > Mesothelin > Biotinylated Human Mesothelin / MSLN (296-580) Protein, His Tag, ultra sensitivity (primary amine labeling)

Biotinylated Human Mesothelin / MSLN (296-580) Protein, His Tag, ultra sensitivity (primary amine labeling)

  • Synonym
    MSLN,Mesothelin,MPF
  • Source
    Biotinylated Human Mesothelin (296-580), His Tag, primary amine labeling (MSN-H8223) is expressed from human HEK293 cells. It contains AA Glu 296 - Gly 580 (Accession # AAH09272). It is the biotinylated form of Human Mesothelin (296-580), His Tag (MSN-H5223).
    Predicted N-terminus: Glu 296
  • Molecular Characterization
    Online(Glu 296 - Gly 580) AAH09272

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 33.0 kDa. The protein migrates as 38-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 1.5-3.5 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Mesothelin (296-580), His Tag, primary amine labeling (Cat. No. MSN-H8223) SDS-PAGE gel

Biotinylated Human Mesothelin (296-580), His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human Mesothelin (296-580), His Tag, primary amine labelingBiotinylated Human Mesothelin (296-580), His Tag, primary amine labeling (Cat. No. MSN-H8223) ELISA bioactivity

Immobilized Anti-Human MSLN MAb at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Mesothelin (296-580), His Tag, primary amine labeling (Cat. No. MSN-H8223) with a linear range of 0.5-8 ng/mL (QC tested).

Evaluation of CAR expression
FACS Analysis of Anti-MSLN CAR Expression
Evaluation of CAR expression

293 cells were transfected with anti-MSLN-scFv and RFP tag. 2e5 of the cells were first stained with B. Biotinylated Human Mesothelin (296-580), His Tag, primary amine labeling (Cat. No. MSN-H8223, 3 µg/mL) and C. Biotinylated Protein Control, followed by FITC Streptavidin. A. Non-transfected 293 cells and C. Biotinylated Protein Control were used as negative control. RFP was used to evaluate CAR (anti-MSLN-scFv) expression and FITC was used to evaluate the binding activity of Biotinylated Human Mesothelin (296-580), His Tag, primary amine labeling (Cat. No. MSN-H8223).

Comments (0)

Order Details

Price(USD) : $290.00

Price(USD) : $1250.00

Promotion & Exhibitions

  • PEGS Boston 2019
  • PepTalk 2019

Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:15 Details
  • Latest Research Phase:Phase ?

Datasheet & Documentation

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Questions & Comments

This web search service is supported by Google Inc.

totop