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Your Position: Home > Immune Checkpoint Proteins > OX40 > Mouse TNFRSF4 /OX40/CD134 Protein,C-Fc Tag

Mouse OX40 / TNFRSF4 / CD134 Protein, Fc Tag

  • Synonym
    TNFRSF4,OX40,CD134,OX40L receptor,ACT35,TXGP1L
  • Source
    Mouse OX40, Fc Tag (OX0-M5259) is expressed from human 293 cells (HEK293). It contains AA Val 20 - Pro 211 (Accession # NP_035789).
    Predicted N-terminus: Val 20
  • Molecular Characterization
    OX40(Val 20 - Pro 211) NP_035789

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 47.9 kDa. The protein migrates as 70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse OX40, Fc Tag (Cat. No. OX0-M5259) SDS-PAGE gel

Mouse OX40, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Mouse OX40, Fc TagMouse OX40, Fc Tag (Cat. No. OX0-M5259) ELISA bioactivity

Immobilized Mouse OX40 Ligand, His Tag (Cat. No. TN4-M5241) at 0.5 μg/mL (100 μL/well) can bind Mouse OX40, Fc Tag (Cat. No. OX0-M5259) with a linear range of 10-16 ng/mL (QC tested).

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