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Your Position: Home > Cytokines > Complement Component C5a > Human Complement Component C5a Protein, Tag Free

Human Complement Component C5a Protein, Tag Free

  • Synonym
    C5a,Complement Component 5a
  • Source
    Human C5a, Tag Free (C5A-H5116) is expressed from E.coli cells. It contains AA Leu 679 - Arg 751 (Accession # P01031).
    Predicted N-terminus: Met
  • Molecular Characterization
    Online(Leu 679 - Arg 751) P01031

    This protein carries no "tag".

    The protein has a calculated MW of 8.3 kDa. The protein migrates as 9 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human C5a, Tag Free (Cat. No. C5A-H5116) SDS-PAGE gel

Human C5a, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human C5a, Tag FreeHuman C5a, Tag Free (Cat. No. C5A-H5116) ELISA bioactivity

Immobilized Human C5a, Tag Free (Cat. No. C5A-H5116) at 2 μg/mL (100 μL/well) can bind Anti-C5a MAb, Human IgG1 with a linear range of 0.8-13 ng/mL (QC tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Human C5a, Tag Free (Cat. No. C5A-H5116) induce N-acetyl-β-D-glucosaminidase release from differentiated U937 cells. The ED50 for this effect is 0.215-0.323 μg/mL (Routinely tested).

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