This protein carries no "tag".
The protein has a calculated MW of 16.5 kDa. The protein migrates as 17 kDa under reducing (R) condition (SDS-PAGE).
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human FGF basic, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 2 μg/mL (100 μL/well) can bind Human Glypican 3, Fc Tag, low endotoxin (Cat. No. GP3-H5258) with a linear range of 4-125 ng/mL (QC tested).
Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) on CM5 Chip can bind Heparin with an affinity constant of 8.37 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Biotinylated Heparin on SA Biosensor, can bind Human FGF basic, Tag Free (Cat. No. BFF-H4117) with an affinity constant of 6.27 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Human FGF basic, Tag Free (Cat. No. BFF-H4117) stimulates proliferation of HUVEC in the range of 0-20 ng/mL. The EC50 for this effect is 0.416-0.630 ng/mL (Routinely tested).
Authors: Jiang, Yongsheng et al.
Journal: Journal of cellular and molecular medicine 2021
Application: Cell-based assay
Authors: Wu YT, et al.
Journal: Sci Rep 2015
Application: Cell Culture
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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