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Your Position: Home > Cytokines > FGF basic > BFF-H4117

Human FGF basic Protein, Tag Free

  • Synonym
    FGF2,BFGF,FGFB,FGF basic,HBGF-2
  • Source
    Human FGF basic, Tag Free (BFF-H4117) is expressed from E.coli cells. It contains AA Pro 143 - Ser 288 (Accession # P09038-4).
    Predicted N-terminus: Met
  • Molecular Characterization
    Online(Pro 143 - Ser 288) P09038-4

    This protein carries no "tag".

    The protein has a calculated MW of 16.5 kDa. The protein migrates as 17 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human FGF basic, Tag Free (Cat. No. BFF-H4117) SDS-PAGE gel

Human FGF basic, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human FGF basic, Tag FreeHuman FGF basic, Tag Free (Cat. No. BFF-H4117) ELISA bioactivity

Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 2 μg/mL (100 μL/well) can bind Human Glypican 3, Fc Tag, low endotoxin (Cat. No. GP3-H5258) with a linear range of 4-125 ng/mL (QC tested).

Human FGF basic, Tag FreeHuman FGF basic, Tag Free (Cat. No. BFF-H4117) ELISA bioactivity

Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 1 μg/mL (100 μL/well) can bind Human Glypican 1, Fc Tag (Cat. No. GP1-H5254) with a linear range of 1-39 ng/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) on CM5 Chip can bind Heparin with an affinity constant of 8.37 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Heparin on SA Biosensor, can bind Human FGF basic, Tag Free (Cat. No. BFF-H4117) with an affinity constant of 1.88 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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