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Your Position: Home > Fc Receptor Proteins > Fc gamma RIIB/C / CD32b/c > CDB-H82E0

Biotinylated Human Fc gamma RIIB/C / CD32b/c Protein, Avitag™,His Tag (SPR & BLI & MALS verified)

  • Synonym
    FCGR2B,C,CD32b,c,FcRII-b,c,Fc-gamma RII-b,c,Fc-gamma-RIIb,c,CD32,FCG2,IGFR2,CDw32
  • Source
    Biotinylated Human CD32b/c, Avitag,His Tag (CDB-H82E0) is expressed from human 293 cells (HEK293). It contains AA Ala 46 - Pro 217 (Accession # P31994-1).
    Predicted N-terminus: Ala 46
  • Molecular Characterization
    Online(Ala 46 - Pro 217) P31994-1

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 23.0 kDa. The protein migrates as 32-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD32b/c, Avitag,His Tag (Cat. No. CDB-H82E0) SDS-PAGE gel

Biotinylated Human CD32b/c, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human CD32b/c, Avitag,His Tag (Cat. No. ) MALS images

The purity of Biotinylated Human CD32b/c, Avitag,His Tag (Cat. No. CDB-H82E0) was more than 90% and the molecular weight of this protein is around 24-36 kDa verified by SEC-MALS.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Biotinylated Human CD32b/c, Avitag,His Tag (Cat. No. CDB-H82E0) captured on Biotin CAP- Series S Sensor Chip can bind Rituximab with an affinity constant of 5 μM as determined in a SPR assay (Biacore T200) (QC tested).

Human_FcRn_Heterodimer_Protein_SPR

Immobilized Biotinylated Human CD32b/c, Avitag,His Tag (Cat. No. CDB-H82E0) on SA Chip, can bind Rituximab with an affinity constant of 4.22 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human CD32b/c, Avitag,His Tag (Cat. No. CDB-H82E0) on SA Biosensor, can bind Rituximab with an affinity constant of 2.7 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Order Details

Price(USD) : $350.00

Price(USD) : $1380.00

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Datasheet & Documentation

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DMF (Drug Master File)

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