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Your Position: Home > Protein > OX40 > OX0-H82F7

Biotinylated Human OX40 Protein, Fc,Avitag™ (MALS verified)

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  • Synonym
    TNFRSF4,OX40,CD134,OX40L receptor,ACT35,TXGP1L
  • Source
    Biotinylated Human OX40, Fc,Avitag(OX0-H82F7) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Ala 216 (Accession # NP_003318 ).
    Predicted N-terminus: Leu 29
  • Molecular Characterization
    OX40 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 48.6 kDa. The protein migrates as 64-67kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris,100 mM Glycine,25 mM Arginine,10 mM NaCl,pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
OX40 SDS-PAGE

Biotinylated Human OX40, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
OX40 MALS images

The purity of Biotinylated Human OX40, Fc,Avitag (Cat. No. OX0-H82F7) is more than 90% and the molecular weight of this protein is around 120-140 kDa verified by SEC-MALS.

Bioactivity-ELISA
 OX40 ELISA

Immobilized Human OX40 Ligand, Fc Tag (Cat. No. OXL-H526x) at 5 μg/mL (100 μL/well) can bind Biotinylated Human OX40, Fc,Avitag (Cat. No. OX0-H82F7) with a linear range of 1-20 ng/mL (QC tested).

Bioactivity-FACS
 OX40 FACS

FACS assay shows that recombinant Biotinylated Human OX40, Fc,Avitag (Cat. No. OX0-H82F7) can bind to 293T cell overexpressing human OX40L. The concentration of OX40 is 0.1 μg/mL (Routinely tested).

  • Background
    Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) is also known as ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD antigen CD134, OX40. OX40 / TNFRSF4 contains four TNFR-Cys repeats. TNFRSF4 is receptor for TNFSF4 / OX40L / GP34 and can interacts with TRAF2, TRAF3 and TRAF5.
  • Clinical and Translational Updates

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