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Your Position: Home > All Other Proteins > PCSK9 > Biotinylated Mouse PCSK9 Protein, Avitag,His Tag

Biotinylated Mouse PCSK9 Protein, Avitag™,His Tag

  • Synonym
    PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
  • Source
    MABSol® Biotinylated Mouse PCSK9, Avitag,His Tag (PC9-M82E1) is expressed from human HEK293 cells. It contains AA Gln 35 - Gln 694 (Accession # AAH38085.1).
    Predicted N-terminus: Gln 35
  • Molecular Characterization
    Online(Gln 35 - Gln 694) AAH38085.1

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 74.9 kDa. The protein migrates as 20 kDa and 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and proteolytic digestion.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse PCSK9, Avitag,His Tag (Cat. No. PC9-M82E1) SDS-PAGE gel

Biotinylated Mouse PCSK9, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Mouse PCSK9, Avitag,His TagBiotinylated Mouse PCSK9, Avitag,His Tag (Cat. No. PC9-M82E1) ELISA bioactivity

Immobilized Mouse LDL R, His Tag (Cat. No. LDR-M52H8) at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse PCSK9, Avitag,His Tag (Cat. No. PC9-M82E1) with a linear range of 2-16 ng/mL (QC tested).

  • Background
    Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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