This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 55.3 kDa. The protein migrates as 80-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>96% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human M-CSF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 96%.
The purity of Human M-CSF R, His Tag (Cat. No. CSR-H5228) was greater than 90% as determined by SEC-HPLC.
Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) immobilized on SA Chip can bind Human M-CSF R, His Tag (Cat. No. CSR-H5228) with an affinity constant of 0.309 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) on SA Biosensor, can bind Human M-CSF R, His Tag (Cat. No. CSR-H5228) with an affinity constant of 378 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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