This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 20.0 kDa. The protein migrates as 21 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) (Cat. No. TNA-H82E3) is more than 90% in HP-SEC, and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Immobilized Humira at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) (Cat. No. TNA-H82E3) with a linear range of 0.4-6 ng/mL (QC tested).
Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) (Cat.No. TNA-H82E3) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is 0.029-0.052 ng/mL (Routinely tested).
Neutralization assay shows that the cytotoxicity effect of Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) (Cat. No. TNA-H82E3) was inhibited by increasing concentration of Adalimumab. The concentration of TNF-alpha used is 1 ng/mL. The IC50 is 7 ng/mL (Routinely tested).
Authors: T Gunde, et al
Journal: US20190153089A1 2019
Authors: Bourquard T, et al.
Journal: J Immunol 2018
Application: Homogeneous time-resolved fluorescence–based competition assay
Authors: Robert Karlsson, et al.
Journal: J Pharmaceut Biomed 2017
Authors: Ji Y, et al.
Journal: J Cell Biochem 2017
Application: Precipitation of TNFR Complexes
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