This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 14.9 kDa. As a result of glycosylation, the protein migrates as 16 kDa and 18-20 kDa under reducing (R) condition, and 30 kDa and 32-40 kDa under non-reducing (NR) condition (SDS-PAGE).
>95% as determined by reduced SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human VEGF121, His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human VEGF121, His Tag (Cat. No. VE1-H5246) is more than 90% and the molecular weight of this protein is around 36-46 kDa verified by SEC-MALS.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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