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Your Position: Home > Immune Checkpoint Proteins > TIGIT > Mouse TIGIT Protein, Fc Tag

Mouse TIGIT Protein, Fc Tag

  • Synonym
    TIGIT,VSIG9,VSTM3
  • Source
    Mouse TIGIT, Fc Tag (TIT-M5257) is expressed from human 293 cells (HEK293). It contains AA Gly 26 - Thr 143 (Accession # NP_001139797).
    Predicted N-terminus: Gly 26
  • Molecular Characterization
    TIGIT(Gly 26 - Thr 143) NP_001139797

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 40 kDa. The protein migrates as 45-52 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Mouse TIGIT, Fc Tag (Cat. No. TIT-M5257) SDS-PAGE gel

Mouse TIGIT, Fc Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Mouse TIGIT, Fc TagMouse TIGIT, Fc Tag (Cat. No. TIT-M5257) ELISA bioactivity

Immobilized Mouse CD155, mouse IgG2a Fc tag (Cat. No. CD5-M5254) at 2 μg/mL (100 μL/well) can bind Mouse TIGIT, Fc Tag (Cat. No. TIT-M5257) with a linear range of 1-16 ng/mL (QC tested).

  • Background
    T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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