This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 27.8 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human PD-L1, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human PD-L1, Avitag,His Tag (Cat. No. PD1-H82E5) is more than 90% and the molecular weight of this protein is around 35-50 kDa verified by SEC-MALS.
Immobilized Biotinylated Human PD-L1, Avitag,His Tag (Cat. No. PD1-H82E5) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) (Cat. No. PD1-H5255) with a linear range of 0.1-2 ng/mL (QC tested).
Authors: Son S, et al
Journal: J Drug Target 2019
Authors: Ganesan A, et al
Journal: Sci Rep 2019
Authors: Yasmina Noubia Abdiche, et al.
Journal: US20160159905A1 2016
Application: Flow Cytometry
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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