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Your Position: Home > All Other Proteins > ICAM-1 > Human ICAM1 CD54 Protein C Fc Tag

Human ICAM-1 / CD54 Protein, Fc Tag

  • Synonym
    ICAM1,BB2,CD54,P3.58
  • Source
    Human ICAM-1, Fc Tag (IC1-H5250) is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Glu 480 (Accession # AAH15969).
    Predicted N-terminus: Gln 28
  • Molecular Characterization
    Online(Gln 28 - Glu 480) AAH15969

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 75.7 kDa. The protein migrates as 90-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >98% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human ICAM-1, Fc Tag (Cat. No. IC1-H5250) SDS-PAGE gel

Human ICAM-1, Fc Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Bioactivity-ELISA
Human ICAM-1, Fc TagHuman ICAM-1, Fc Tag (Cat. No. IC1-H5250) ELISA bioactivity

Immobilized Human ITGALB2, His tag at 5 μg/mL (100 μL/well) can bind Human ICAM-1, Fc Tag (Cat. No. IC1-H5250) with a linear range of 0.082-1.28 μg/mL (Routinely tested).

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