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Your Position: Home > Receptor Tyrosine Kinases > M-CSF R > Mouse M-CSF R / CSF1R / CD115 Protein, Fc Tag

Mouse M-CSF R / CSF1R / CD115 Protein, Fc Tag, low endotoxin

  • Synonym
    CSF1R,C-FMS,CD115,CSFR,FIM2,FMS,M-CSFR
  • Source
    Mouse M-CSF R, Fc Tag, low endotoxin (CSR-M5256) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Ser 511 (Accession # NP_001032948).
    Predicted N-terminus: Ala 20
  • Molecular Characterization
    M-CSF R(Ala 20 - Ser 511) NP_001032948

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 81.8 kDa. As a result of glycosylation, the protein migrates as 100-130 kDa under reducing (R) condition, and 260 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse M-CSF R, Fc Tag, low endotoxin (Cat. No. CSR-M5256) SDS-PAGE gel

Mouse M-CSF R, Fc Tag, low endotoxin on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Mouse M-CSF R, Fc Tag, low endotoxinMouse M-CSF R, Fc Tag, low endotoxin (Cat. No. CSR-M5256) ELISA bioactivity

Immobilized Mouse M-CSF Protein at 2μg/mL (100 μL/well) can bind Mouse M-CSF R, Fc Tag (Cat. No. CSR-M5256) with a linear range of 0.3-12.8 ng/mL (Routinely tested).

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