This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 7.8 kDa. The protein migrates as 12 kDa and 14-18 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>80% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human BCMA Protein, His Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human BCMA Protein, His Tag (HPLC-verified) was greater than 80% as determined by SEC-HPLC.
Immobilized Human BCMA Protein, His Tag (Cat. No. BCA-H522y) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human BCMA Antibody, Human IgG1 with a linear range of 0.2-3 ng/mL (Routinely tested).
Anti-Human BCMA MAb (human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 13.0 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Anti-BCMA Neutralizing Antibody, Mouse IgG1 captured on CM5 chip via anti-mouse antibodies surface can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 0.234 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Anti-Human BCMA MAb (human IgG1) on Protein A Biosensor, can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 29.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human APRIL, Fc Tag (Cat. No. APL-H5267) on Protein A Biosensor, can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 10.9 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human BAFF, Fc Tag (Cat. No. BAF-H4268) on Protein A Biosensor, can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 0.64 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
2e5 of BCMA-CAR-293 cells transfected with anti-BCMA-scFv were stained with 100 μL of 0.3 μg/mL of Human BCMA / TNFRSF17 Protein,His Tag DMF Filed (Cat. No. BCA-H522y) and negative control protein respectively, washed and then followed by FITC anti-6xHis tag antibody and analyzed with FACS. (Routinely tested).
FULLY HUMANIZED ANTI-B CELL MATURATION ANTIGEN (BCMA) SINGLE-CHAIN ANTIBODY AND USE THEREOF
Authors: Li Zhiyuan, Zhai Tianhang, et al.
Journal: US20200339699A1 2020
Application: BLI
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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