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Your Position: Home > Immune Checkpoint Proteins > CD27 > TN7-H82F6

Biotinylated Human CD27 / TNFRSF7 Protein, Fc,Avitag™

  • Synonym
    CD27,TNFRSF7,S152,T14,Tp55
  • Source
    Biotinylated Human CD27, Fc,Avitag (TN7-H82F6) is expressed from human 293 cells (HEK293). It contains AA Thr 21 - Ile 192 (Accession # NP_001233).
    Predicted N-terminus: Thr 21
  • Molecular Characterization
    Online(Thr 21 - Ile 192) NP_001233

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 47.7 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD27, Fc,Avitag (Cat. No. TN7-H82F6) SDS-PAGE gel

Biotinylated Human CD27, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human CD27, Fc,AvitagBiotinylated Human CD27, Fc,Avitag (Cat. No. TN7-H82F6) ELISA bioactivity

Immobilized Human CD27 Ligand, Fc Tag (Cat. No. TN7-H526x) at 10 μg/mL (100 μL/well) can bind Biotinylated Human CD27, Fc,Avitag (Cat. No. TN7-H82F6) with a linear range of 5-156 ng/mL (QC tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Flow Cytometry assay shows that Biotinylated Human CD27, Fc,Avitag (Cat. No. TN7-H82F6) can bind to Raji cells Surface Human CD70. The concentration of CD27 used is 10 μg/mL (Routinely tested).

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Price(USD) : $350.00

Price(USD) : $1380.00

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