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Your Position: Home > All Other Proteins > Protein G > Recombinant Protein G Protein, His Tag

Recombinant Protein G Protein, His Tag

  • Synonym
    RPG,Protein G
  • Source
    Recombinant Protein G, His Tag, also known as Recombinant Staphylococcus Aureus protein G148, was expressed in E.Coli at ACRObiosystems.
  • Molecular Characterization
    Online()

    Recombinant Protein G, His Tag is fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. The reducing (R) protein migrates as a 29 kDa polypeptide. Protein G Protein can bind to human IgG1 Fc.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >98% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 100 mM NaH2PO4, pH8.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Recombinant Protein G, His Tag (Cat. No. RPG-S3140) SDS-PAGE gel

Recombinant Protein G, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Bioactivity-ELISA
Recombinant Protein G, His TagRecombinant Protein G, His Tag (Cat. No. RPG-S3140) ELISA bioactivity

Immobilized Recombinant Protein G, His Tag (Cat. No. RPG-S3140) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IgG1 Fc, Avitag (Cat. No. IG1-H8213) with a linear range of 0.01-0.313 μg/mL (Routinely tested).

  • Background
    Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from this recombinant form of Protein G.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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