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Your Position: Home > Immune Checkpoint Proteins > LAG-3 > LA3-H5255

Human LAG3 / CD223 Protein, Fc Tag (MALS Verified)

  • Synonym
    LAG3,CD223,FDC
  • Source
    Human LAG-3, Fc Tag (LA3-H5255) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1).
    Predicted N-terminus: Leu 23
  • Molecular Characterization
    Online(Leu 23 - Leu 450) P18627-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 72.8 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human LAG-3, Fc Tag (Cat. No. LA3-H5255) SDS-PAGE gel

Human LAG-3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human LAG-3, Fc Tag (Cat. No. ) MALS images

The purity of Human LAG-3, Fc Tag (Cat. No. LA3-H5255) was more than 95% and the molecular weight of this protein is around 215-240 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human LAG-3, Fc TagHuman LAG-3, Fc Tag (Cat. No. LA3-H5255) ELISA bioactivity

Immobilized Human LAG-3, Fc Tag (Cat. No. LA3-H5255) at 2 μg/mL (100 μL/well) can bind Madarex LAG-3 MAb with a linear range of 0.4-3 ng/mL (QC tested).

  • Background
    Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, which belongs to immunoglobulin (Ig) superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. LAG3 /CD223 involved in lymphocyte activation. LAG3 /CD223 binds to HLA class-II antigens.
  • References
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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