Human ANGPTL7 / CDT6 Protein

INTRODUCTION

QC & Assay Protocol

Citation & Background

Synonym
ANGPTL7,CDT6
Source
Human ANGPTL7, His Tag (AN7-H52H5) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Pro 346 (Accession # O43827-1).
Predicted N-terminus: Gln 27
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Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 39 kDa. The protein migrates as 38-40 kDa and 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>85% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
1. 4-8^°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Human ANGPTL7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

Background
Angiopoietin-like 7 (ANGPTL7) is also known as Corneal-Derived Transcript 6 (CDT6), and is a secreted glycoprotein that is structurally related to the angiopoietins. It is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor. Recently, ANGPTL proteins, including ANGPTL-2, -3, -4, -5 and -7, are identified as growth factors in combination with SCF, Thrombopoietin, IGF-II and FGF acidic that enhance the expansion and engraftment of human and mouse hematopoietic stem cells.
References
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