Bulk order Acrobiosystems for Chinese

Chinese

A-Z

2019-nCoV | CD24 | CD73 | Trimer | CD19 | PD-1

There is no goods in the shopping cart !

My Cart

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9

Home
Product & Service

Products by Category

Recombinant proteins and more
Immune Checkpoint ProteinsHot Targets of CAR-T Cell TherapyNew Biosimilar Drug TargetsHot Anti-idiotypic AntibodyHot Fc Receptor Proteins Llama IgG Fc Fusion ProteinNew Bio-Markers & CD Antigens ActiveMax -Best Cytokines Viral Proteins Enzymes Antibodies All Recombinant Proteins
Services

Innovation Lab ServicesNew

Pre-labeled proteins
Biotinylated protein collectionHot FITC & PE labeled ProteinsNew Fluorescent fusion proteins
Kits
ELISA Assay Kits for Quantitative Analysis of Therapeutic Antibodies in Serum SamplesNew Inhibitor Screening Kits
Others
HPLC-verified Proteins

Hot Products

CD73 - A novel target for immunotherapyNew mAb for Anti-CD20 CAR Detection - Flow Cytometry ValidatedNew Siglec-15 - Validated by Cell-based Assay BLI/ SPR/ ELISANew Integrin - Integrin ProteinsNew CD20 - Full Length ProteinExclusive FGL1 - Fibrinogen-like protein 1New PD1 - PD1 Pathway TargetsHot CD19 - High Affinity CD19, Designed for Detection of Anti-CD19 CAR ExpressionNew CD3E - Biotinylated CD3E productsHot BCMA - B-Cell Maturation Antigen TIGIT - TIGIT Signaling Axis CD47 - Leukocyte Surface Antigen CD47 LAG3 - Lymphocyte-activation gene 3 ROR - Recombinant ROR1 & ROR2
Support

How to Order Shipping & Payments Global Distributors Testimonials Download Brochures Download COA
Application

Quantitative Analysis of mAb in Serum Evaluation of CAR Expression
X-LAB
Company

About Us Join Us Contact Us
Activity

News Exhibitions Promotion

Your Position: Home > Immune Checkpoint Proteins > LILRB4 > CDK-C5227

Cynomolgus LILRB4 / CD85k / ILT3 Protein, His Tag

INTRODUCTION

QC & Assay Protocol

Citation & Background

Synonym
LILRB4,ILT3,LIR5,CD85K,HM18
Source
Cynomolgus LILRB4, His Tag (CDK-C5227) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Glu 259 (Accession # XP_015297198.1).
Predicted N-terminus: Gln 22
Request for sequence
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 28.0 kDa. The protein migrates as 34-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
1. -20^°C to -70^°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Cynomolgus LILRB4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA

Cynomolgus LILRB4, His TagCynomolgus LILRB4, His Tag (Cat. No. CDK-C5227) ELISA bioactivity

Immobilized Cynomolgus LILRB4, His Tag (Cat. No. CDK-C5227) at 2 μg/mL (100 μL/well) can bind Human ANGPTL7, His Tag (Cat. No. AN7-H52H5) with a linear range of 8-125 ng/mL (QC tested).

Protocol

Background
Leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4) is also known as CD85 antigen-like family member K (CD85K), Immunoglobulin-like transcript 3 (ILT-3), Leukocyte immunoglobulin-like receptor 5 (LIR-5), Monocyte inhibitory receptor HM18, which belongs to the leukocyte immunoglobulin-like receptor (LIR) family. LILRB4 / CD85K contains 2 Ig-like C2-type (immunoglobulin-like) domains. CD85K is detected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells. LILRB4 / CD85K is receptor for class I MHC antigens. CD85K recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles, involved in the down-regulation of the immune response and the development of tolerance. LILRB4 interferes with TNFRSF5-signaling and NF-kappa-B up-regulation and inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
References
- (1) Cella M., et al., 1997, J. Exp. Med. 185:1743-1751.
- (2) Chang C.C., et al., 2002, Nat. Immunol. 3:237-243.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.