This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 35.9 kDa. The protein migrates as 38-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human Mesothelin (296-580), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human Mesothelin (296-580), His,Avitag (Cat. No. MSN-H82E9) was more than 85% and the molecular weight of this protein is around 45-55 kDa verified by SEC-MALS.
293 cells were transfected with Anti-MSLN-scFv and RFP tag. 2e5 of the cells were first stained with B. Biotinylated Human Mesothelin (296-580), His,Avitag (Cat. No. MSN-H82E9, 3 µg/mL) and C. Biotinylated Protein Control, followed by FITC Streptavidin. A. Non-transfected 293 cells and C. Biotinylated Protein Control were used as negative control. RFP was used to evaluate CAR (Anti-MSLN-scFv) expression and FITC was used to evaluate the binding activity of Biotinylated Human Mesothelin (296-580), His,Avitag (Cat. No. MSN-H82E9).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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