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Your Position: Home > Protein > LILRB2 > LI2-H82F5

Biotinylated Human LILRB2 / CD85d / ILT4 Protein, Fc,Avitag™ (MALS verified)

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  • Synonym
    LILRB2,ILT4,LIR2,MIR10,CD85d
  • Source
    Biotinylated Human LILRB2, Fc,Avitag(LI2-H82F5) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Val 461 (Accession # AAH36827.1).
    Predicted N-terminus: Gln 22
  • Molecular Characterization
    LILRB2 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 76.2 kDa. The protein migrates as 90-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
LILRB2 SDS-PAGE

Biotinylated Human LILRB2, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
LILRB2 MALS images

The purity of Biotinylated Human LILRB2, Fc,Avitag (Cat. No. LI2-H82F5) is more than 90% and the molecular weight of this protein is around 165-201 kDa verified by SEC-MALS.

Bioactivity-ELISA
 LILRB2 ELISA

Immobilized Anti-LILRB2 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human LILRB2, Fc,Avitag (Cat. No. LI2-H82F5) with a linear range of 1-16 ng/mL (QC tested).

  • Background
    Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2) is also known as CD85 antigen-like family member D (CD85d), Immunoglobulin-like transcript 4 (ILT-4), Monocyte / macrophage immunoglobulin-like receptor 10 (MIR-10), which is a member of the the subfamily B class of LIR receptors. LILRB2 is receptor for class I MHC antigens. LILRB2 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB2 competes with CD8A for binding to class I MHC antigens. LILRB2 / CD85d inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
  • Clinical and Translational Updates

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ETD of in-stock products: 2 business days

Price(USD) : $368.00

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