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Your Position: Home > Receptor Tyrosine Kinases > M-CSF R > Biotinylated Mouse M-CSF R / CSF1R / CD115 Protein, Avitag,His Tag

Biotinylated Mouse M-CSF R / CSF1R / CD115 Protein, Avitag™,His Tag

INTRODUCTION

QC & Assay Protocol

Citation & Background

Synonym
CSF1R,C-FMS,CD115,CSFR,FIM2,FMS,M-CSFR
Source
Biotinylated Mouse M-CSF R, Avitag,His Tag (CSR-M82E8) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Ser 511 (Accession # P09581-1).
Predicted N-terminus: Ala 20
Request for sequence
Molecular Characterization
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 58.4 kDa. The protein migrates as 78-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
1. 4-8^°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Biotinylated Mouse M-CSF R, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA

Biotinylated Mouse M-CSF R, Avitag,His TagBiotinylated Mouse M-CSF R, Avitag,His Tag (Cat. No. CSR-M82E8) ELISA bioactivity

Immobilized Human M-CSF, His Tag (Cat. No. MCF-H5247) at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse M-CSF R, Avitag,His Tag (Cat. No. CSR-M82E8) with a linear range of 0.625-10 μg/mL (QC tested).

Protocol

Background
Colony stimulating factor 1 receptor (CSF1R) is also known as macrophage colony-stimulating factor receptor (M-CSFR), CD115 Cluster of Differentiation 115 (CD115), C-FMS, CSFR, FIM2, FMS, and is a member of the typeⅢ subfamily of receptor tyrosine kinases (RTKs). CSF1R is a receptor for a cytokine called colony stimulating factor 1, The protein encoded by the CSFR1 gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most, if not all, of the biological effects of this cytokine. Ligand binding activates CSFR1 through a process of oligomerization and transphosphorylation . Mutations in CSF1R are associated with chronic myelomonocytic leukemia and type M4 acute myeloblastic leukemia. Increased levels of CSF1R1 are found in microglia in Alzheimer's disease and after brain injuries. The increased receptor expression causes microglia to become more active. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.
References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.