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Product Details
Synonyms
EGFR, ERBB, ERBB1, HER1, PIG61, mENA
Source
Biotinylated Human EGF R, His,Avitag (EGR-H82E3) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).
Predicted N-terminus: Leu 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 72.3 kDa. The protein migrates as 100-115 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human EGF R, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human EGF R, His,Avitag (Cat. No. EGR-H82E3) is more than 95% and the molecular weight of this protein is around 80-110 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Biotinylated Human EGF R, His,Avitag (Cat. No. EGR-H82E3) with a linear range of 2-6 ng/mL (QC tested).
Protocol
Immobilized Human EGF, Tag Free at 10 μg/mL (100 μL/well) can bind Biotinylated Human EGF R, His,Avitag (Cat. No. EGR-H82E3) with a linear range of 0.156-5 μg/mL (Routinely tested).
Protocol
Bioactivity-TR-FRET
In this TR-FRET assay, Streptavidin Protein Europium chelate (for TR-FRET) (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-Human IgG Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA02) is used as the Acceptor. Biotinylated Human EGF R, His,Avitag (Cat. No. EGR-H82E3), at 1.25 ng/well, can bind to the Amivantamab with a linear detection range of 8-0.008 μg/mL (Routinely tested).
In this TR-FRET assay, Streptavidin Protein Europium chelate (for TR-FRET) (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-Human IgG Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA02) is used as the Acceptor. Biotinylated Human EGF R, His,Avitag (Cat. No. EGR-H82E3), at 1.25 ng/well, can bind to the Cetuximab with a linear detection range of 8-0.001 μg/mL. (Routinely tested)
Bridging ELISA
Validated with Bispecific Antibody.
Immobilized Human HGF R, His Tag (Cat. No. MET-H5227) at 5 μg/mL, add increasing concentrations of Amivantamab and then add Biotinylated Human EGF R, His,Avitag (Cat. No. EGR-H82E3) at 5 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4.88 ng/mL (Routinely tested).
Evaluation of CAR expression
FACS Analysis of biotinylated EGFR binding cell surface anti-EGFR scFv
Cells were transfected with anti-EGFR scFv and cultured for 3 days. Three days post-transfection, 1e6 cells were stained for anti-EGFR scFv with Biotinylated Human EGFR, His,Avitag (Cat. No. EGR-H82E3) followed by PE-conjugated streptavidin. Flow Cytometry assay shows that Biotinylated Human EGFR, His,Avitag (Cat. No. EGR-H82E3) can bind to cells expressing anti-EGFR scFv. The concentration of EGFR used is 0.8 µg/mL. (Data are kindly provided by Guangzhou Bio-gene Technology Co. Ltd.)
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Background
Recent Advances
Drug Development Progress- English Name：
Epidermal growth factor receptor erbB1
- Category：
- Approved Drugs：
39 Details
- Drugs in Clinical Trials：
206 Details
- Highest Development Stage：
Approved
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