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Your Position: Home > Receptor Tyrosine Kinases > Her2 > Recombinant Human Her2 / ErbB2 / NEU Protein

Human Her2 / ErbB2 Protein, Fc Tag

  • Synonym
    ERBB2,CD340,HER-2/neu,HER2,MLN19,NEU,NGL,TKR1
  • Source
    Human Her2, Fc Tag (HE2-H5253) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # P04626-1).
    Predicted N-terminus: Thr 23
  • Molecular Characterization
    Her2(Thr 23 - Thr 652) P04626-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 96.0 kDa. The protein migrates as 125-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    The formulation may be subject to change. Please contact us if you need to confirm the exact formulation of the current lot

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Her2, Fc Tag (Cat. No. HE2-H5253) SDS-PAGE gel

Human Her2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human Her2, Fc TagHuman Her2, Fc Tag (Cat. No. HE2-H5253) ELISA bioactivity

Immobilized Human Her2, Fc Tag (Cat. No. HE2-H5253) at 0.05 μg/mL (100 μL/well) can bind Trastuzumab with a linear range of 0.39-6.25 ng/mL (QC tested).

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