This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 58.6 kDa. The protein migrates as 66-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Mesothelin (296-580), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human Mesothelin (296-580), Fc Tag(Cat. No. MSN-H5253) was more than 90% and the molecular weight of this protein is around 130-150 kDa verified by SEC-MALS.
293 cells were transfected with anti-MSLN-scFv and RFP tag. 2e5 of the cells were first stained with B. Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253, 1 µg/ml) and C. Fc Tag Fusion Protein Control, followed by FITC anti-human IgG Fc. A. Non-transfected 293 cells and C. Fc Tag Fusion Protein Control were used as negative control. RFP was used to evaluate CAR (anti-MSLN-scFv) expression and FITC was used to evaluate the binding activity of Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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