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Your Position: Home > All Other Proteins > Mesothelin > MSN-H5253

Human Mesothelin / MSLN (296-580) Protein, Fc Tag (MALS verified)

  • Synonym
    MSLN,Mesothelin,MPF
  • Source
    Human Mesothelin (296-580), Fc Tag (MSN-H5253) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1).
    Predicted N-terminus: Glu 296
  • Molecular Characterization
    Online(Glu 296 - Gly 580) AAH09272.1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 58.6 kDa. The protein migrates as 66-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253) SDS-PAGE gel

Human Mesothelin (296-580), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human Mesothelin (296-580), Fc Tag (Cat. No. ) MALS images

The purity of Human Mesothelin (296-580), Fc Tag(Cat. No. MSN-H5253) was more than 90% and the molecular weight of this protein is around 130-150 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human Mesothelin (296-580), Fc TagHuman Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253) ELISA bioactivity

Immobilized Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253) at 0.5 μg/mL (100 μL/well) can bind Anti-Human MSLN Mab with a linear range of 0.1-3 ng/mL (QC tested).

Evaluation of CAR expression
FACS Analysis of Anti-MSLN CAR Expression
Evaluation of CAR expression

293 cells were transfected with anti-MSLN-scFv and RFP tag. 2e5 of the cells were first stained with B. Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253, 1 µg/ml) and C. Fc Tag Fusion Protein Control, followed by FITC anti-human IgG Fc. A. Non-transfected 293 cells and C. Fc Tag Fusion Protein Control were used as negative control. RFP was used to evaluate CAR (anti-MSLN-scFv) expression and FITC was used to evaluate the binding activity of Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253).

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