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Your Position: Home > Immune Checkpoint Proteins > 4‑1BB Ligand > Biotinylated Human 4-1BB Ligand / TNFSF9 Protein, Fc,Avitag

Biotinylated Human 4-1BB Ligand / TNFSF9 Protein, Fc,Avitag™

  • Synonym
    4‑1BB Ligand,TNFSF9,CD137L
  • Source
    Biotinylated Human 4-1BB Ligand, Fc,Avitag (41L-H82F9) is expressed from human 293 cells (HEK293). It contains AA Ala 50 - Glu 254 (Accession # P41273-1).
    Predicted N-terminus: Ala 50
  • Molecular Characterization
    Online(Ala 50 - Glu 254) P41273-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 50.2 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human 4-1BB Ligand, Fc,Avitag (Cat. No. 41L-H82F9) SDS-PAGE gel

Biotinylated Human 4-1BB Ligand, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human 4-1BB Ligand, Fc,AvitagBiotinylated Human 4-1BB Ligand, Fc,Avitag (Cat. No. 41L-H82F9) ELISA bioactivity

Immobilized Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) at 0.1 μg/mL (100 μL/well) can bind Biotinylated Human 4-1BB Ligand, Fc,Avitag (Cat. No. 41L-H82F9) with a linear range of 0.4-13 ng/mL (QC tested).

  • Background
    Tumor necrosis factor ligand superfamily member 9 (4-1BBL) is also known as 4-1BB ligand, CD137L or TNFSF9, which is a cytokine that binds to TNFRSF9. 4-1BBL is the high affinity ligand of 4-1BB. 4-1BBL induces the proliferation of activated peripheral blood T-cells. Also, 4-1BBL may have a role in activation-induced cell death (AICD). Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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