This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
The protein has a calculated MW of 9.6 kDa. The protein migrates as 12 kDa and 15-17 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human BCMA, His,Avitag™ on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) at 5 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, His,Avitag (Cat. No. BCA-H82E4) with a linear range of 0.01-0.313 μg/mL (QC tested).
Loaded Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) on Protein A Biosensor, can bind Biotinylated Human BCMA, His,Avitag (Cat. No. BCA-H82E4) with an affinity constant of 0.69 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
293 cells were transfected with anti-BCMA-scFv and RFP tag. 2e5 of the cells were first stained with B. Biotinylated Human BCMA, His,Avitag (Cat. No. BCA-H82E4, 1 μg/mL) and C. Biotinylated Protein Control, followed by FITC Streptavidin. A. Non-transfected 293 cells and C. Biotinylated Protein Control were used as negative control. RFP was used to evaluate CAR (anti-BCMA-scFv) expression and FITC was used to evaluate the binding activity of Biotinylated Human BCMA, His,Avitag (Cat. No. BCA-H82E4).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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