This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 9.6 kDa. The protein migrates as 12 kDa and 15-17 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human BCMA, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) at 5 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, His,Avitag (Cat. No. BCA-H82E4) with a linear range of 0.01-0.313 μg/mL (QC tested).
Loaded Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) on Protein A Biosensor, can bind Biotinylated Human BCMA, His,Avitag (Cat. No. BCA-H82E4) with an affinity constant of 0.69 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
2e5 of anti-BCMA CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Human BCMA, His,Avitag (Cat. No. BCA-H82E4) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).
Authors: A Martin, JE Chatterton, MB Pires.
Journal: US20200370014A1 2020
Application: Flow cytometry
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