This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 73.0 kDa. The protein migrates as 85-95 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human Her2, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Biotinylated Human Her2, His,Avitag (Cat. No. HE2-H82E2) immobilized on SA Chip can bind Herceptin® with an affinity constant of 17 pM as determined in a SPR assay (Biacore T200) (Routinely tested).
2e5 of Anti-Her2 CAR-293 were stained with 100 μL of 1 μg/mL of Biotinylated Human Her2 Protein, His,Avitag™ (Cat. No. HE2-H82E2) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Authors: Chatterjee Sohang , et al
Journal: US20200149032A1 2020
Application: Flow Cytometry
Authors: NR Sundah, et al
Journal: Nat Biomed Eng 2019
Authors: YM Kim, et al
Journal: US20180360986A1 2019
Authors: Lobb Roy, et al.
Journal: US20180142035A1 2018
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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