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Your Position: Home > All Other Proteins > TSLP R > Human TSLP R Protein, Fc Tag

Human TSLP R Protein, Fc Tag

  • Synonym
    TSLP R,CRLF2,IL-XR,ILXR,p2RY8/CRLF2 fusion,TSLP receptor,TSLPR,CRL2,CRLF2Y,Cytokine receptor-like 2
  • Source
    Human TSLP R, Fc Tag (TSR-H525a) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Lys 231 (Accession # Q9HC73-1).
    Predicted N-terminus: Gly 25
  • Molecular Characterization
    Online(Gly 25 - Lys 231) Q9HC73-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 50.4 kDa. The protein migrates as 62-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human TSLP R, Fc Tag (Cat. No. TSR-H525a) SDS-PAGE gel

Human TSLP R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human TSLP R, Fc TagHuman TSLP R, Fc Tag (Cat. No. TSR-H525a) ELISA bioactivity

Immobilized Human TSLP R, Fc Tag (Cat. No. TSR-H525a) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) with a linear range of 4-31 ng/mL (QC tested).

Human TSLP R, Fc TagHuman TSLP R, Fc Tag (Cat. No. TSR-H525a) ELISA bioactivity

Biotinylated Human TSLP (R127A, R130A), His,Avitag (Cat. No. TSP-H82E0) immobilized at 2 μg/mL (100 μL/well) via precoated 5 μg/mL (100 μL/well) of Human TSLP R, Fc Tag (Cat. No. TSR-H525a), can bind can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. IL7-H5258) with a linear range of 1-20 ng/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Captured Human TSLP, His Tag (Cat. No. TSP-H52Hb) on CM5 Chip via anti-His antibody, can bind Human TSLP R, Fc Tag (Cat. No. TSR-H525a) with an affinity constant of 1.81 nM as determined in SPR assay (Biacore T200) (Routinely tested).

Human_FcRn_Heterodimer_Protein_SPR

Captured Human TSLP (R127A, R130A), His Tag (Cat. No. TSP-H52Ha) on CM5 Chip via anti-His antibody, can bind Human TSLP R, Fc Tag (Cat. No. TSR-H525a) with an affinity constant of 4.22 nM as determined in SPR assay (Biacore T200) (Routinely tested).

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