This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 16.8 kDa. The protein migrates as 8 kDa and 14-20 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human TSLP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human TSLP, His Tag (Cat. No. TSP-H52Hb) at 0.5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human TSLP Antibody, Human IgG2 with a linear range of 0.2-4 ng/mL (QC tested).
Captured Human TSLP, His Tag (Cat. No. TSP-H52Hb) on CM5 Chip via anti-His antibody, can bind Human TSLP R, Fc Tag (Cat. No. TSR-H525a) with an affinity constant of 1.81 nM as determined in SPR assay (Biacore T200) (Routinely tested).
Monoclonal Anti-Human TSLP Antibody (Human IgG2) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human TSLP Protein, His Tag (Cat. No. TSP-H52Hb) with an affinity constant of 5.47 pM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Monoclonal Anti-Human TSLP Antibody, Human IgG2 on AHC Biosensor, can bind Human TSLP, His Tag (Cat. No. TSP-H52Hb) with an affinity constant of 0.263 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Response to human TSLP protein (Fold).
The TSLPR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human TSLP protein (AcroBiosystems, Cat. No. TSP-H52Hb). The max induction fold was approximately 45.15 (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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