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Your Position: Home > Immune Checkpoint Proteins > GITR > Human GITR / TNFRSF18 Protein, Mouse IgG2a Fc Tag, low endotoxin

Human GITR / TNFRSF18 Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    AITR,GITR,TNFRSF18,CD357
  • Source
    Human GITR, Mouse IgG2a Fc Tag, low endotoxin (GIR-H525a) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Glu 161 (Accession # Q9Y5U5-1).
    Predicted N-terminus: Gln 26
  • Molecular Characterization
    GITR(Gln 26 - Glu 161) Q9Y5U5-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 41.4 kDa. The protein migrates as 46-49 kDa and 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human GITR, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. GIR-H525a) SDS-PAGE gel

Human GITR, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human GITR, Mouse IgG2a Fc Tag, low endotoxinHuman GITR, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. GIR-H525a) ELISA bioactivity

Immobilized Human GITR Ligand, Fc Tag (Cat. No. GIL-H526a) at 5 μg/mL (100 μL/well) can bind Human GITR, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. GIR-H525a) with a linear range of 0.039-0.313 μg/mL (QC tested).

  • Background
    Glucocorticoid-induced TNFR-related protein (GITR) is also known as Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), activation-inducible TNFR family receptor (AITR), CD antigen CD357, which is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is receptor for TNFSF18, which seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR also mediated NF-kappa-B activation via the TRAF2/NIK pathway.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $300.00

Price(USD) : $1980.00

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