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Your Position: Home > Recombinant Proteins > CD48 > Human CD48 / SLAMF2 Protein, Mouse IgG2a Fc Tag, low endotoxin

Human CD48 / SLAMF2 Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    CD48,BCM1,SLAMF2,BLAST,BLAST1,MEM-102,TCT.1,BCM-1,SLAMF-2,BLAST-1
  • Source
    Human CD48, Mouse IgG2a Fc Tag, low endotoxin (BC1-H5258) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Ser 220 (Accession # P09326-1).
    Predicted N-terminus: Gln 27
  • Molecular Characterization
    CD48(Gln 27 - Ser 220) P09326-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 49.2 kDa. The protein migrates as 64-70 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human CD48, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. BC1-H5258) SDS-PAGE gel

Human CD48, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human CD48, Mouse IgG2a Fc Tag, low endotoxinHuman CD48, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. BC1-H5258) ELISA bioactivity

Immobilized Human 2B4, Fc Tag (Cat. No. 2B4-H5255) at 2 μg/mL (100 μL/well) can bind Human CD48, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. BC1-H5258) with a linear range of 2-31 ng/mL (QC tested).

  • Background
    CD48 antigen is also known as Signaling lymphocytic activation molecule 2, SLAM family member 2 (SLAMF2), Leukocyte antigen MEM-102, BCM1 surface antigen, B-lymphocyte activation marker BLAST-1. CD48 is a 65 kDa GPI-linked protein which belongs to the CD2 subfamily of immunoglobulin superfamily molecules. CD48 antigen contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domain. CD48 interacts with CD244 in an heterophilic manner. CD48 is ligand for CD2. CD48 might facilitate interaction between activated lymphocytes. CD48 is probably involved in regulating T-cell activation.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $300.00

Price(USD) : $1980.00

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