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This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™)
The protein has a calculated MW of 56.9 kDa. The protein migrates as 75-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human CA125, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human Mesothelin, His Tag (Cat. No. MSN-H5223) at 10 μg/mL (100 μL/well) can bind Biotinylated Human CA125, Fc,Avitag (Cat. No. CA5-H82F4) with a linear range of 2-78 ng/mL (QC tested).
Human Mesothelin (296-580), His Tag, premium grade (Cat. No. MSN-H522a) immobilized on CM5 Chip can bind Biotinylated Human CA125, Fc,Avitag (Cat. No. CA5-H82F4) with an affinity constant of 0.66 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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